PacBio Sequel Systems at Novogene
The PacBio Sequel System is the newest SMRT sequencing platform that provides ultra-long sequencing reads with high consensus accuracy, uniform coverage, and simultaneous epigenetic characterization at single-molecule resolution. The PacBio Sequel System provides 7x more throughput, more scalability, and lower sequencing costs than the PacBio RS II System, while maintaining the same benefits. With the addition of 20 PacBio Sequel Systems that Novogene acquired in early 2017, Novogene now has the world’s largest SMRT sequencing capacity.
The PacBio Sequel System provides unmatched sequencing depths for genomes, transcriptomes and epigenomes. Novogene not only offers PacBio Sequel-based de novo sequencing services, but also provides combined services using various platforms, including Illumina HiSeq, PacBio Sequel, and 10X Genomics Chromium, to match the needs of researchers. Please consult our scientists for the best sequencing strategy to achieve the most comprehensive de novo assembly view for your genome of interest. Novogene’s superior high performance computing and data analysis platform enables high-standard complex genome assembly and fast data analysis.
SMRT Sequencing Advantages
• Long average read lengths: average > 10kb, some reads > 60kb
• High consensus accuracy: >99.999% (QV50) consensus accuracy
• Uniform coverage: no amplification required and even coverage across GC content
• Simultaneous epigenetic characterization: directly detect DNA modifications using polymerase kinetics
• Single-molecule resolution: Circular Consensus Sequencing (CCS) produces high intra-molecular consensus accuracy
PacBio Sequel System Applications
A. De novo Sequencing and Assembly of Plant and Animal Genomes
The SMRT sequencing provides uniform coverage and delivers more complete genomes without amplification. The ultra-long reads and uniform coverage also solve the sequencing and assembly difficulties encountered with highly repetitive regions, transposon regions, and highly variable regions, and leads to longer contigs and scaffolds. Thus, the Sequel system is ideal for large genome projects, including human, plant, and animal genomes, as this system can rapidly and cost-effectively generate high-quality de novo assemblies of whole-genome.
Below are some common strategies that we recommend for de novo sequencing of plant and animal genomes based on individual project needs:
B. De novo Sequencing and Assembly of Microbial Genomes
The ultra-long reads from PacBio Sequel System can cover the entire repeat regions of bacterial genomes, providing complete (zero gap) sequences. Assemblies of chromosomal scale also become possible for fungi with this system, and the full chromosomal sequences generated allow individual strains and species to be compared, for example, in evolutionary studies. See below for some recommended strategies.
C. Isoform Sequencing (Iso-Seq) and Full-length Transcripts Generation
Iso-Seq sequencing, based on the extraordinarily long reads available with the SMRT technology, provides full-length reads spanning entire transcript isoforms from the polyA-tail to the 5′ end without the need for assembly. Iso-Seq is ideal for improving annotations in reference genomes, characterizing gene isoforms, alternative splicing and gene fusion events, as well as complementing the short / incomplete transcripts for species without a reference genome.