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Pre-made Library Sequencing

Introduction to Pre-made Library Sequencing

With our world-leading, powerful, and sophisticated sequencing platforms (Illumina, Pacbio, and Oxford nanopore platform), we provide sequencing services for the pre-made libraries at different read lengths and capacities to suit any cost, project scale, and turnaround.

Novogene accepts customers’ prepared libraries for sequencing. We have a variety of sequencing platforms that offer a variety of sequencing read length, flux, and time cycle options. We directly sequenced qualified libraries provided by customers and produced high-quality sequencing data, thus providing data basis for bioinformatics analysis by customers. Novogene has a professional and efficient operations team to ensure customer project cycle and data quality, to provide customers with fast and easy sequencing services. We combine scientific scheme design, strict quality control management, rich project experience and high-quality project service to ensure that every link can be excellently completed to facilitate scientific research. With our world-leading capacity, including the Illumina, Pacbio and Oxford nanopore platform, we provide sequencing service for the pre-made library at highly competitive and cost-effective prices.

Benefits of Pre-made Library Sequencing

  • State-of-the-art NGS technologies: Novogene is a top-brand in sequencing capacity using state-of-the-art technology, including Illumina HiSeq X, NovaSeq 6000 Systems, Pacbio sequel II and Oxford Nanopore promethION Beta.
  • Sequencing strategy: Novogene employs PE150, PE250, PE50, SE50, and other customized set-ups.
  • Extensive experience: We take pride in completing over 2000+ sequencing projects, and our data has been published in many high-profile journals.

Pre-made Library Sequencing Specifications:
Sample Requirements

Platform Data Amount Volume Requirement* Library concentration
HiSeq System, Paired-end 150 Lane Sequencing ≥ 20μL/lane (additionaL 10μL for one more lane) ≥ 0.5 ng/μL, quantified by Qubit® 2.0 (Life Technologies)

2 nM-30 nM, quantified by Q-PCR

NovaSeq System, Paired-end 150 < 20 G ≥ 15μL
20G ≤X ≤ 100G ≥ 25 μL
100G <X<400G ≥ 50 μL
Lane Sequencing ≥ 50 μL/lane (additional 40uL for one more lane)
NovaSeq System, Paired-end 250, Paired-end 50, Single-end 50 X ≤ 20M ≥ 15 μL
20M<X ≤ 50M ≥ 25 μL
50M< X <150M* ≥ 50 μL
Lane Sequencing ≥ 100 μL/lane (additional 100uL for one more lane)

*Notes:
High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.

Pre-made Library Sequencing Specifications:
Illumina Sequencing

 

Platform Read Length Output
NovaSeq 6000 PE50 400M /lane
800M /flowcell
SE50 400M /lane
800M / flowcell
PE250 400M /lane
800M / flowcell
PE150 800G /lane
3.2T / flowcell
HiSeq X-ten PE150 110G/lane
880G/ flowcell

Novogene Workflow of Pre-made Library Sequencing

For self-constructed libraries provided by the customers, quality analysis is performed initially before sequencing to ensure the quality of the data. The qualified libraries are fed into Illumina sequencers after pooling according to their effective concentrations and expected data volumes.