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Pre-made Library Sequencing

Introduction to Pre-made Library Sequencing

We offer tailored sequencing services using cutting-edge sequencing platforms, including Illumina, PacBio, and Oxford Nanopore. Our comprehensive solutions accommodate libraries prepared by customers, providing various read lengths and capacities to meet diverse project needs. Whether you require a cost-effective option, a large-scale endeavor, or a rapid turnaround, our services can align with your specific requirements.

Novogene offers sequencing services for customer-prepared, ready-to-sequence libraries. Our diverse portfolio of sequencing platforms provides flexibility in read lengths, throughput, and turnaround times. We sequence various compatible library types provided by customers, delivering high-quality sequencing data to serve as a foundation for bioinformatics analysis.

Our experienced team are committed to providing customers efficient and seamless sequencing services, integrating scientific expertise, rigorous quality control, extensive project experience and efficient operations to ensure excellence at every stage, facilitating scientific research.

Why Choose Novogene?

  • State-of-the-art NGS Capacity: Novogene is a top-brand in sequencing capacity using state-of-the-art technology, including Illumina NovaSeqX Plus systems, NovaSeq 6000, Pacbio Revio, sequel II/ IIe and Oxford Nanopore promethION Beta.
  • Flexible sequencing strategy: Novogene employs PE150, PE250, PE50, SE50, and other customized sequencing modes.
  • Trusted Expertise: Rely on the expertise of Novogene’s experienced team to guide you through every step of your sample processing.
  • Rapid Turnaround Time: Novogene offers the fastest turnaround time upfront so you can benefit from quicker sample processing from QC to data release, allowing you to accelerate your project timelines.

Pre-made Library Sequencing Specifications:
Sample Requirements

Sequencing Strategy Sequencing Platform Sequencing Data Amount Volume Requirement Library Concentration

PE150

NovaSeq 6000 S4

< 30 Gb

≥ 15 μL

≥ 2 ng/μL, quantified by Qubit® 2.0 (Life Technologies)

2 nM-30 nM, quantified by

Q-PCR

30 Gb ≤ X ≤ 100 Gb

≥ 25 μL

100 Gb < X ≤ 800 Gb

≥ 50 μL

NovaSeq 6000 S4

Lane sequencing (800G/lane)

≥ 50 μL (additional 40 μL for one more lane)

NovaSeq X Plus 10B

Lane sequencing (375G/lane)

≥ 50 μL (additional 40 μL for one more lane)

NovaSeq X Plus 25B

Lane sequencing (1000G/lane)

≥ 130 μL per lane

SE50 PE50

NovaSeq 6000 SP

Flow cell sequencing

(2 lanes per flow cell)

≥ 100 μL per lane

PE250

NovaSeq 6000 SP

Lane sequencing

≥ 100 μL per lane

Pre-made Library Sequencing Specifications:
Illumina Sequencing

 

Platform Sequencing Strategy Data Output

NovaSeq X Plus 10B

PE150

375 G /lane
3.0 T / flowcell

NovaSeq X Plus 25B

PE150

1000 G /lane
7.6 T / flowcell

NovaSeq 6000 S4

PE150

800 G /lane
3.2 T / flowcell

NovaSeq 6000 SP

PE50

400 M /lane
800 M /flowcell

SE50

400 M /lane
800 M / flowcell

PE250

400 M /lane
800 M / flowcell

Novogene Workflow of Pre-made Library Sequencing

For self-constructed libraries provided by the customers, quality analysis is performed initially before sequencing to ensure the quality of the data. The qualified libraries are fed into Illumina sequencers after pooling according to their effective concentrations and expected data volumes.