De novo Sequencing
De novo sequencing generates an initial genomic sequence of a particular organism without a reference genome. Services can be applied to research of animals, plants, and microorganisms, including phylogenetic studies, analysis of species diversity, genetic markers, and other genomic research.
Technology
Sequencing Platform
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ILLUMINA
NovaSeq 6000
System specifications:80Gb - 6Tb output range, 1.6 - 40 B paired end reads per run, and PE150, PE250, PE50, etc.
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ILLUMINA
HiSeq X
System specifications:1.6 – 1.8 Tb output range, 5.3 – 6 B reads per run, and 2 x150 bp max read length.
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PacBio
PacBio Sequel II/IIe System
System specifications:N50>15kb, read lengths up to 25kb (CCS), >99.999% (QV50) consensus accuracy, coverage across high-GC/repeat regions. HiFi reads for PacBio Sequel IIe only.
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Nanopore
Nanopore PromethION
System specifications:Ultra-long reads up to 2Mb, high yields for large genomes, REAL real-time and accessible for flexible budgets.
