De novo Sequencing
Technology
Sequencing Platform
Nanopore PromethION System
• Ultra-long reads – up to 2 Mb
• High yields for large genomes
• Direct DNA/RNA sequencing without extra labeling
• REAL Real-time
• Accessible for flexible budgets
PacBio Sequel System
• Long average read lengths: average > 10kb, some reads > 60kb
• High consensus accuracy: >99.999% (QV50) consensus accuracy
• Uniform coverage: no amplification required and even coverage across GC content
• Simultaneous epigenetic characterization: directly detect DNA modifications using polymerase kinetics
• Single-molecule resolution: Circular Consensus Sequencing (CCS) produces high intra-molecular consensus accuracy
HiSeq 4000
System specifications: 105 – 1500 Gb output range, 2.1 M – 5 B reads per run, and 2 x 150 bp max read length
NovaSeq 6000
System specifications: 167Gb – 6Tb output range, 1.6 – 20 B reads per run, and 2 x 150 bp max read length
