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Whole Genome Bisulfite Sequencing

Introduction to Whole Genome Bisulfite Sequencing

DNA methylation at the C5 position of cytosine plays a crucial role in gene expression and chromatin remodeling. Perturbations in methylation patterns are associated with tumorigenesis, neurodegenerative diseases and neurological disorders. Bioinformatic analysis of methylome is widely used in various research areas, including studies on gene regulation, cell differentiation, embryogenesis, aging, occurrence and development of disease, phenotypic diversity and evolution in plants and animals.

Novogene provides whole genome bisulfite sequencing (WGBS) to efficiently identify methylated cytosines on a genome-wide scale with a single nucleotide resolution. The deep reach of the methylome is imperative for understanding gene expression and multiple biological processes that are subject to epigenetic regulation.

How does Whole Bisulfite Genome Sequencing apply?

Novogene delivers high-quality data and publication-ready analysis results of WGBS services, to faciliate research including:

  • Profiling methylation patterns at CG, CHG and CHH sites with single nucleotide resolution
  • Identifying differentially methylated sites associated with experimental treatments or sample conditions
  • Understanding mechanisms of cell differentiation or tissue development on the basis of methylation profiles
  • Early diagnosis of diseases and cancers through the detection of DNA hypermethylation or hypomethylation

Benefits of Novogene WGBS Service

  • Hundreds of projects successfully have been completed with well guaranteed data output and highly professional bioinformatics analysis
  • Ultra-low input DNA and high bisulfite conversion rate are available in methylation library preparation
  • Industry-standard software (such as Bismark) and mature in-house pipelines are used for mapping and comprehensive bioinformatics analysis
  • Customized association analysis can explore correlations between methylation and gene expression

WGBS Specifications: DNA Sample Requirements

Sample Type Required Amount Purity
Genomic DNA ≥ 200 ng OD260/280=1.8-2.0

Note: Sample amounts are listed for reference only. Download the Service Specifications or Sample Requirements to learn more. For detailed information, please contact us with your customized requests.

WGBS Specifications: Sequenicng & Analysis

Platform Type Illumina NovaSeq 6000
Read Length Paired-end 150bp
Sequencing Depth ≥ 30× coverage for the species with reference genome
Standard Data Analysis
  • Data Quality Control
  • mCs detection, methylation level calculation
  • Methylation level and frequency distribution
  • Differentially Methylated Site (DMS) detection
  • Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation
  • Function enrichment of DMR-associated genes and DMP-associated genes
  • Visualization of BS seq data
  • Comparative analysis (among samples)
  • Note: Sequencing parameters and analysis contents displayed are only for reference. Download the Service Specifications to learn more. For detailed information, please contact us with your customized requests.

    Project Workflow of Novogene WGBS Service

    The Novogene WGBS service comprises of four steps. Sample preparation is followed by library preparation, sequencing and bioinformatics analysis. To construct a methylation library, DNA fragmentation performs initially, and sodium bisulfite converts the unmethylated cytosines into uracils. Libraries are then sequenced using Illumina PE150 and eventually data extraction and bioinformatics analysis take steps.

    In order to ensure the accuracy and reliability of sequencing data, Novogene audits every experimental step strictly by quality control and ensures high-quality data output fundamentally, from DNA sampling to obtaining final data report. Obtaining high-quality data is the premise to ensure that bioinformatics analysis is correct, comprehensive and credible.

    Featured Publications using Novogene WGBS Service

    Whole Genome Bisulfite Sequencing (WGBS) is a powerful method to identify individually methylated cytosines on a genome-wide scale. The deep reach of the methylome (including methylated cytosine detection), the promoter detection of differentially methylated sites and regions, and related functional analysis of the Differentially Methylated Region (DMR) and Differentially Methylated Promoter (DMP), are all essential for understanding gene expression and other processes that are subject to epigenetic regulation. Here summarizes a list of publications that used Novogene WGBS service.

    Distribution of Genome Coverage

    Novogene WGBS Distribution of Genome Coverage


    Methylation Level Distribution on Whole Genome

    Novogene WGBS Methylation Level Distribution on Whole Genome


    Heatmap Analysis for Methylation Levels of Gene Functional Region

    Novogene WGBS Heatmap Analysis for Methylation Levels of Gene Functional Region


    Methylation Level Distribution at Functional Genetic Elements

    Novogene WGBS Methylation Level Distribution at Functional Genetic Elements


    Circos Plot for DMR Condition in three contexts (CG, CHG, CHH)

    Novogene WGBS Circos Plot for DMR Condition in three contexts (CG, CHG, CHH)


    Diagram of GO Enrichment

    Novogene WGBS GO Enrichment