Eight high-quality genomes reveal pan-genome architecture and ecotype differentiation of Brassica napus
Year : 2020 | Category : Brassica napus, Applications: De novo Sequencing, GWAS, Pan Genome
Brassica napus is an allotetraploid plant with a complex genome. According to the low temperature requirement of flowering, Brassica napus can be divided into three ecological types: winter rape (WORs), spring rape (SORs) and semi-winter rape (SWORs). There are a wide range of genomic and phenotypic variations in different germplasm and ecotypes of Brassica napus. Therefore, it is necessary to carry out pan-genome research. In this study, 8 high-quality reference genomes of brassica napus were published, and a pan-genome and uniform genetic index were constructed based on 9 reference genomes. A large number of SNPS and presence/absence variants (PAV) were identified by a pan-genomic comparative analysis. In combination with the brassica napus nest correlation plot (bn-nam) population, the structural variation that controls the length, grain weight and flowering time of siliaca napus was revealed. Further analysis revealed that PAV of the three FLC genes was closely related to flowering time and ecotypic differentiation. This study provides resources for analyzing the genome structure and accelerating genetic improvement of brassica napus l.
Figure: Features of the B. napus genome