Eukaryotic mRNA-seq Sample Preparation — Getting Qualified Total RNA Samples
RNA is a single-stranded nucleic acid polymer of the four nucleotides A, C, G, and U, joined through a backbone of alternating phosphate and ribose residues, converting information from DNA into proteins or to serve direct roles in cells, like rRNA, tRNA, etc. Among all kinds of RNAs in cells, messenger RNA (mRNA) is a molecular that carries codes from the DNA in the nucleus to the sites of protein synthesis in the cytoplasm (the ribosomes).
Novogene’s mRNA-Seq, based on state-of-the-art Illumina NovaSeq platforms, is commonly used to profile the snapshot of mRNA or even the entire transcriptome at a given moment in time. It offers comprehensive solutions for analyzing gene expression and differential gene expression among sample groups, as well as for identification of gene structure variations, such as alternative splicing, gene fusion events, etc.
High-quality total RNA as starting material for an mRNA-seq project is vital to the successful mining of bioinformatics information from the sequencing data. Novogene conducts stringent Quality Control to help you select high-quality total RNA samples for sequencing, avoiding any potential issues downstream. What’s more, in this webinar we also share with you our extensive experience as a guideline in sample preparation from tissues.