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PROTEOMICS

Service Overview
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NovoFocus NSCLC CDxProteomics is a comprehensive and systematic analysis of protein identification and quantification from a macroscopic perspective.Together with the other “omics” technologies, such as genomics and transcriptomics, it assists in expounding the identity of proteins to reveal their expression, structure, functions, interactions and modifications.

Proteomics plays a crucial role in drug development. Characterizing the proteins of a cell, tissue or organism is highly useful in the identification of candidate biomarkers, bacterial antigens and possible immunohistochemistry markers targeted by the immune response and related to infectious or neoplastic diseases. In addition, by detecting the alteration of expression patterns in different diseases or in response to different treatments, proteomics can also explain distinct patient reactions during clinical intervention.

Novogene applies mass spectrometry (MS) technology by using the Thermo Q ExactiveTM HF-X mass spectrometer to achieve accurate mass determination and protein characterization, providing customers with unsurpassed data and excellent service.

The Novogene Advantage

  • Comprehensive Analysis: Including quantitative, qualitative, post-translational modification and in-depth protein profiling.
  • High Performance Platform: The Thermo Q ExactiveTM HF-X mass spectrometer guarantees high sensitivity and excellent stability in analysis quality.
  • Extraordinary Informatics Expertise: Cutting-edge bioinformatics pipeline with internationally recognized best-in-class software, proving customers with publication-ready data.

Proteomics Service Overview

Qualitative Proteomics

The qualitative analysis includes identification of proteins, protein interactions and protein modifications after collection from gel, as well as identification of the full spectrum proteome from tissue or blood.

Service Overview

Post-translational Modification Proteomics

Post-translational modification (PTM) of a protein is a biochemical process that increases the functional diversity of the proteome by enzymatically adding functional groups to
the protein following protein biosynthesis. Common modification types include phosphorylation, acetylation and ubiquitination, which change the molecular mass accordingly.
Mass spectrometry allows for protein modification to be quantified, thus enabling post-translational modifications to be analyzed qualitatively and quantitatively.

In-depth Proteomics

Analysis method

Result Display

Protein quantitative analysis


  • Volcano diagrams of differential expression proteins

  • Hierarchical clustering heatmap

  • Protein domain enrichment histogram

  • KEGG enrichment scatter plot of differential expression proteins

  • GO enrichment DAG

  • Protein-protein interaction networks

Correlation analysis with transcriptome

  • Venn diagram of expression regulation
  • Correlation analysis of expression level
  • GO enrichment correlation analysis
  • GO enrichment clustering heatmap

Post-translational modification analysis

  • Motif analysis
  • Clustering heatmap of phosphorylated kinases

Examples of Publications Using Novogene’s Expertise

JOURNAL TITLE
Microbiome, 5:14 (2017) Gut microbiota dysbiosis contributes to the development of hypertension
Nature Communications,10:1639 (2019) KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
For Research Use Only. The content provided herein may relate to products that have not been approved for clinical use by the regulatory authorities of where this document is circulated. The application of such products should thus be limited to research only. The content of this document is subject to change without notice. © 2018 Novogene Co., LTD. All rights reserved. All trademarks are the property of Novogene Co., LTD and its subsidiaries unless otherwise specified.