Accurately characterizing the diverse landscape of transcript isoforms generates key information for understanding the functional biology of genomes, but assembling complete transcripts, has been challenging. Isoform sequencing (Iso-Seq™), based on the extraordinarily long reads available with PacBio SMRT technology, provides full-length reads spanning entire transcript isoforms from the polyA-tail to the 5′ end, with no assembly needed! Iso-Seq is ideal for improving annotations in reference genomes, for characterizing gene isoforms, alternative splicing and gene fusion events, as well as for complementing the short/incomplete transcripts for species without a reference genome.
With the largest Illumina and SMRT sequencing capacity in the world, Novogene offers complete solutions for full-length transcript sequencing using combined Illumina and PacBio Sequel platforms. Illumina short reads are further mapped to the full-length transcripts generated from Sequel for error correction and for more accurate quantification analysis. Our experienced bioinformatics experts work closely with customers to provide standard
and customized data analysis and publication-ready results for species with and without a reference genome.
We also offer Illumina-based mRNA, lncRNA, circular RNA, and small RNA sequencing services. Contact us to learn more.
The Novogene Advantage
Largest sequencing capacity: We have the largest Illumina and PacBio sequencing capacities in the world, allowing us to provide high quality data, fast turnaround, and affordable prices.
Comprehensive data analysis: We use widely accepted mainstream software and mature in-house pipeline to discover novel transcripts, differential expressions, and function annotations
Project Workflow
Sample Requirements
AMOUNT (QUBIT®)
CONCENTRATION (QUBIT®)
A260/280
A260/230
28S/18S
Nc/Qc
RNA INTEGRITY NUMBER (AGILENT 2100)
PURITY (NANODROP™/AGAROSE GEL)
≥ 5 μg
≥ 300ng/ul
1.8~2.2
≥ 1.8
≥ 1.5
≤ 2.0
≥ 9, with the smooth baseline
No degradation, no contamination
Sequencing Strategy
Library Type: Full-length cDNA library with equimolar mix of selected 4k+ fragments and non-selected fragments
Sequencing Platform: PacBio Sequel system
Sequencing Depth: ≥ 15G raw data/sample
Recommended Sequencing Depth
≥ 15 G Raw Data / Sample
Turnaround Time
Within 7 weeks from project verification to data releasing without bioinformatic analysis
Analysis Pipeline
Figure 1: pipline for ISO-SEQ analysis
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PacBio Sequel Systems at Novogene
