Leading Edge Genomic Services & Solutions

Iso-Seq on PacBio

Service Overview
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Iso-Seq on PacBio PacBio Sequel Systems at Novogene
Accurately characterizing the diverse landscape of transcript isoforms generates key information for understanding the functional biology of genomes, but assembling complete transcripts, has been challenging. Isoform sequencing (Iso-Seq™), based on the extraordinarily long reads available with PacBio SMRT technology, provides full-length reads spanning entire transcript isoforms from the polyA-tail to the 5′ end, with no assembly needed! Iso-Seq is ideal for improving annotations in reference genomes, for characterizing gene isoforms, alternative splicing and gene fusion events, as well as for complementing the short/incomplete transcripts for species without a reference genome. With the largest Illumina and SMRT sequencing capacity in the world, Novogene offers complete solutions for full-length transcript sequencing using combined Illumina and PacBio Sequel platforms. Illumina short reads are further mapped to the full-length transcripts generated from Sequel for error correction and for more accurate quantification analysis. Our experienced bioinformatics experts work closely with customers to provide standard and customized data analysis and publication-ready results for species with and without a reference genome. We also offer Illumina-based mRNA, lncRNA, circular RNA, and small RNA sequencing services. Contact us to learn more.

The Novogene Advantage

  • Largest sequencing capacity: We have the largest Illumina and PacBio sequencing capacities in the world, allowing us to provide high quality data, fast turnaround, and affordable prices.
  • Comprehensive data analysis: We use widely accepted mainstream software and mature in-house pipeline to discover novel transcripts, differential expressions, and function annotations

Project Workflow

Sample Requirements

≥ 5 μg ≥ 300ng/ul 1.8~2.2 ≥ 1.8 ≥ 1.5 ≤ 2.0 ≥ 9, with the smooth baseline No degradation, no contamination

Sequencing Strategy

  • Library Type: Full-length cDNA library with equimolar mix of selected 4k+ fragments and non-selected fragments
  • Sequencing Platform: PacBio Sequel system
  • Sequencing Depth: ≥ 15G raw data/sample

Recommended Sequencing Depth

  • ≥ 15 G Raw Data / Sample

Turnaround Time

  • Within 7 weeks from project verification to data releasing without bioinformatic analysis

Analysis Pipeline

  • Figure 1: pipline for ISO-SEQ analysis