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Our Single Cell Sequencing Solutions

Making Single Cell Technologies Accessible and Simpler

At Novogene, we believe that single cell technologies should be easily accessible for spurring advances in scientific discoveries. We offer the single cell sequencing solutions on the 10x Genomics Chromium System and Illumina platforms. The highlight of this technique enables massive transcriptional profiling of thousands of individual cells at single cell resolution.

Download our Solution Brochure to learn more.

We offer the following single cell sequencing services to our AMEA (Asia Pacific, Middle East and Africa) customers:

  • Single Cell Gene Expression
  • Single Cell Immuno Profiling
  • Single Cell Multiome ATAC
  • Single Cell ATAC
  • Spatial Gene Expression
Our Service Overview and Turnaround Time

We like to be an extension of your laboratory to manage your single cell sequencing experiments. Whether it’s complete end-to-end services or on-site technical support of your single cell projects, we are here to help keep you efficient and productive in achieving your research goal.

Complete end-to-end services

We have a dedicated single cell sequencing facility in Singapore to carry out your projects from:

  • Project design
  • Picking up single cell suspension from your site*
  • Cell barcoding
  • Library preparation
  • Sequencing
  • Data analysis using 10x Genomics Cell Ranger Software and our bioinformatics pipelines.

*Applicable for Singapore only.

On-site technical support

Our experts can also lend their service on-site to perform your single cell projects. As the nature of each project differs, please contact your local representative.


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Project Design

Samples

Construction

Sequencing

Bioinformatics

Schedule a meeting with us to discuss the planning of your project design and cell preparation.

Prepared samples must comply strictly with our samples requirements. We can perform/assist cell counting. We will arrange the pick up of your single cell suspension from your site.

We can manage your single-cell library preparation that includes – GEM Generation and barcoding, Post GEM-RT Cleanup and cDNA amplification, 3’ Gene expression library construction.

High-throughput single-cell RNA sequencing on the Illumina NovaSeq 6000 platform.

Bioinformatics analysis using 10x Genomics Cell Ranger Software and our bioinformatics pipelines.

Analysis examples of the t-SNE and UMAP plots:


 
 
Analysis examples of expression pattern of marker genes:
Sample Requirements and Turnaround Time

The below table shows the projected turnaround time frame of our single cell sequencing services upon receipt of your sample. Cryopreservation, fixation and nuclei isolation from archival samples are alternative preparation methods we could accept.

As the nature of each project differs, please contact your local representative for a detailed discussion and TAT outcome of your project.

Sample Type Amount Remarks TAT (< 4 samples)
Single cells/ nuclei suspension
prepared from cell lines and/or solid tissue
At least 50,000 cells Activity ≥ 80%,
diameter: 5-40um
OD260/280=1.8-2.0;
4 weeks
(w/o data analysis)
Publications

View some of the recent publications that were supported by Novogene solutions below and more at our website.